AIDS Research and Human Retroviruses · 2003 · 24 citations · 13 references
Life SciencesGeneticsMolecular GeneticsMolecular VirologyPhylogeneticsVirologyHiv EpidemiologyHiv SurveillancePhylogenetic EpidemiologyViral GeneticsViral GenomicsVirus ClassificationGenetic Transmission PatternsGenetic Variant DetectionHigh-risk Population PrevalenceHigh-risk Population SurveillanceHiv PrevalencePhylogenetic Character AnalysisPhylogenetic Diversity AnalysisVirus Genotypes
The HIV-1 prevalence among bar and hotel workers in Tanzania suggests they are a high-risk group for HIV-1 infection. We determined the HIV-1 subtype of 3'-p24/5'-p7 gag and C2-C5 env sequences from 40 individuals representing this population in Moshi. Genetic patterns composed of A(gag)-A(env), C(gag)-C(env), and D(gag)-D(env) were found in 19 (48.0%), 8 (20.0%), and 3 (8.0%) samples, respectively. The remaining 10 samples (25%) had different subtypes in gag and env, indicative of intersubtype recombinants. Among these recombinants, two contained sequences from HIV-1 subsubtype A2, a new genetic variant in Tanzania. Five bar and hotel workers may have been infected with viruses from a common source, based on phylogenetic analysis. The information obtained by surveillance of HIV-1 subtypes in a high-risk population should be useful in the design and evaluation of behavioral, therapeutic, and vaccine trial interventions aimed at reducing HIV-1 transmission.
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