Preparation and Evaluation of Slurry-Packed Liquid Chromatography Microcolumns with Inner Diameters from 12 to 33 μm

Abstract

Fused silica capillary liquid chromatography columns with inner diameters between 12 and 33 microns were slurry packed with 5 microns octadecylsilane-modified silica particles. Column efficiencies and van Deemter coefficients were compared. A linear decrease of the A term as column diameter was decreased was the most significant contributor to a lower overall plate height at the optimum velocity.