Journal of Dental Research · 2011 · 59 citations · 30 references
Life SciencesMolecular BiologyOncologyGene ExpressionGenomicsMolecular OncologyCancer BiomarkersCancer GenomicsCancer TranscriptomicsMolecular Tumor ProfilingOral Cancer BiomarkersTranscriptomic ProfilingAmeloblast Gene ExpressionCluster AnalysisLaser Capturing MicrodissectionMolecular Prognostic MarkersParaffin-embedded Tissue StainingPrognostic Tumor MarkersRna Expression Signature AnalysisTumor-specific Biomarkers
Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets.
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Medulloblastoma Growth Inhibition by Hedgehog Pathway Blockade
David M. Berman, Sunil Karhadkar, Andrew R. Hallahan et al. · Science · 2002 · 794 citations
Medicine, Oncology, Pharmacology +17
Universal Reference RNA as a standard for microarray experiments
Natalia Novoradovskaya, Michael L. Whitfield, Lee S Basehore et al. · BMC Genomics · 2004 · 167 citations · Full text