Reproduction in Domestic Animals · 1991 · 177 citations · 7 references
SpermatogenesisSperm PopulationsFertilityGeneticsReproductive HealthGynecologyPreimplantation Genetic TestingSemen AnalysisReproductive BiologyFertilisationY‐bearing SpermReproductive PhysiologyFemale InfertilityMale InfertilityGametogenesisSex PreselectionPublic HealthInfertilitySperm BiologyMature GiltsGameteGenetic VariationPopulation GeneticsFlow Sorted X‐Animal ReproductionDevelopmental BiologyEvolutionary BiologyMedicineSperm Population
Contents: Flow cytometric sorting of X and Y chromosome‐bearing sperm into separate populations, followed by surgical insemination into the isthmus of the oviduct of mature gilts, produced litters with phenotypic sex ratios skewed in the direction of male or female according to the predicted sex of the sperm population inseminated. The skewing of the sex ratio of the offspring was predicted from flow cytometric DNA reanalysis of flow sorted sperm populations. Gilts inseminated with intact, viable flow‐sorted X‐bearing sperm produced litters of off spring that were 74% female (P < .01). Gilts inseminated with intact, viable flow‐sorted Y‐bearing sperm gave off spring that were 68% male (P < .04). These results validate the use of DNA as a marker for pre‐selecting the sex of offspring when used in conjunction with flow cytometric sorting of sperm .
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L. A. Johnson, J. G. Aalbers, HJ.G. Grooten · Reproduction in Domestic Animals · 1988 · 167 citations
Flow sorting of X and Y chromosome‐bearing spermatozoa into two populations
Lawrence A. Johnson, J. P. Flook, M. V. Look et al. · Gamete Research · 1987 · 126 citations