Analytical Chemistry · 2000 · 68 citations · 9 references
Natural SciencesBiochemistryPharmaceutical AnalysisAnalytical ChemistryCancer PharmacologyMass Spectrometric AnalysisBioanalytical ChemistryBioanalytical Method ValidationInstrumental AnalysisMass SpectrometryMetabolite ExtractionMetabolite QuantificationCardiac GlycosidesCollision-induced DissociationDetection Limit AssessmentLiquid Chromatography-mass Spectrometry AnalysisMass Spectrometric DetectionMass Spectrometry CharacterizationMethod Detection LimitTandem Mass Spectrometry Analysis
An HPLC/MS/MS method has been developed for the characterization and quantification of the cardiac glycosides oleandrin, odoroside, neritaloside and the aglycone oleandrigenin, all contained in a patented-hot-water extract of Nerium oleander L (Anvirzel). Qualitative analysis of such extracts was achieved using a hybrid tandem quadrupole time-of-flight (QqTOF) mass spectrometer. Collision-induced dissociation (CID) mass spectra of oleandrin, oleandrigenin, odoroside, and neritaloside were obtained with greater than 5 ppm mass accuracy and resolution routinely in excess of 8000 (fwhm). The detection limit for oleandrin of 20 pg (injected) was realized when the precursor-to-product ion transition, m/z 577 --> 373, was monitored. We have also applied the analytical method to the determination of oleandrin, oleandrigenin, neritaloside, and odoroside in human plasma following an intramuscular injection of Anvirzel.
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Bio-active cardenolides from the leaves of Nerium oleander
Sabira Begum, Bina S. Siddiqui, Razia Sultana et al. · Phytochemistry · 1999 · 76 citations
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