Anti‐C3d Antiglobulin Reagents. II. Preparation of an Antiglobulin Serum Monospecific for C3d

Abstract

Three approaches are described to the preparation of a potent antiglobulin reagent monospecific for the C3d component of C3: 1) attempts to absorb out the anti‐C3c component of a bisperific anti‐C3c, C3d serum, 2) attempts to isolate by affinity chromatography purified C3d to be used for animal immunization, 3) animal immunization with a relatively crude filtration fraction of aged serum which contained C3d but lacked C3c. Problems encountered with the first two approaches are described and discussed. Preparation of a potent anti‐C3d serum by the third approach is documented, as are methods employed to assess the specificity and potency of the reagent.