Enzymatic L-Arginine and L-Lysine Determination Using a Carbonate Ion Selective Membrane Electrode

Abstract

Abstract Manual and automated flow methods for the determination of 1-arginine and 1-lysine using decarboxylase enzymes and a carbonate ion selective membrane electrode are described. Excellent results are obtained in the millimolar concentration range for aqueous samples having physiological saline levels. The method could be utilized for the simple determination of specific amino acids in biological samples.