Drug Metabolism Letters · 2008 · 25 citations · 0 references
MedicinePharmacologyToxicologyBiochemical PharmacologyHepatic PharmacokineticsMetabolomicsCytochrome P450 EnzymesCytochrome P450-mediated MetabolismEnzyme InductionHepatic Drug MetabolismHepatic MetabolismCytochrome P450 2C9Cytochrome P450 Drug MetabolismCytochrome P450 Enzyme ActivityCytochrome P450 Enzyme InductionCytochrome P450 Human Liver MicrosomesCytochrome P450 Metabolism AssaysInduction In HepatocytesPrediction Of Induction Potential
A fast and sensitive method was developed for the assessment of CYP induction in human hepatocytes cultured in 96-well plates. The effects on CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, and CYP3A4 were examined by means of mass spectrometry and the well-known cocktail strategy. The performance of the method was tested by using prototypic inducers such as methylcolanthrene, phenobarbital and rifampicin. The method was shown to be robust and predictable for the in vitro induction potential studies of drugs.