Chromosome banding techniques for morphologically classified cells

Abstract

This report describes staining techniques for chromosome banding and sister chromatid exchanges (SCEs) suited to a method that allows simultaneous analysis of cell morphology and karyotype. Mitotic cells are first identified by either cytochemical staining or immunologic methods. The preparations are then destained and treated with acid fixative. For G- and C-banding, the cells are incubated overnight at room temperature in Søorensen buffer and then stained with Giemsa. To demonstrate SCEs, the cells are fluorescent stained before being stained with Giemsa.