Proceedings of the National Academy of Sciences · 1995 · 24 citations · 16 references
Life SciencesGeneticsMolecular GeneticsGenetic EngineeringGenomicsRecombinationGene MappingGenetic Interaction MappingGenetic MappingMutation AnalysisMutation ScreeningScreening Of MutantsHomologous Recombination CloningMutational MappingPhenotype-genotype LinkingRecombination MappingYeast Genetic MappingYeast Hybridization GeneticsYeast Mutagenesis
An efficient method for mapping mutations is described in which hybrid genes, derived partly from mutant and partly from wild-type DNA, are obtained in vivo by homologous recombination of multiple fragments. The recombinants are formed in a strain in which their phenotypes are immediately apparent. This method was developed to identify changes that disrupt protein-protein interactions demonstrable by the two-hybrid system in yeast. However, it can be extended to any system where recombination is possible, provided an assay is available to distinguish between mutant and wild-type phenotypes.
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A novel genetic system to detect protein–protein interactions
Stanley Fields, Ok-Kyu Song · Nature · 1989 · 6.1K citations