Acid phosphatase isoenzymes of <i>Brassica oleracea</i> seedlings and their application to sib testing in F₁ hybrids

Abstract

SUMMARY Crude extracts from cotyledons of 2‐day‐old seedlings were separated by electrophoresis on polyacrylamide gels and stained for acid phosphatase. At least 22 bands were recognised, distributed over seven zones. Analysis of zone 1 allowed estimation of sib frequencies in 12 out of 19 hybrid cultivars. By applying either seedling zone 1 or acp ‐1 seed acid phosphatase analysis, sib estimates could be made in 15 of these hybrids. It is suggested that this technique offers a practical and rapid means of estimating sib frequencies in commercial seed stocks.