An Enzymatic Assay of 2,3 - Diphosphoglycerate in Blood

Abstract

2,3-diphosphoglycerate was estimated by an enzymatic method, based on the catalytical effect of 2,3-diphosphoglycerate on the phosphoglycerate mutase reaction, which was coupled with that of lactate dehydrogenase by adding ADP, enolase, pyruvate kinase, and NADH to the system. The activity was estimated from the decrease in NADH-concentration. Samples were obtained by diluting whole blood 100 times with oxygenated water, without any other pretreatment of the blood. The normal range of 2,3-diphosphoglycerate concentration in blood from normal individuals was determined.