Plant regeneration in <i>Actinidia polygama</i> Miq. by leaf, stem, and petiole culture with zeatin, and from stem-derived calli on low-sucrose medium

Abstract

We examined the effect of zeatin on the formation of shoot buds from explants and callus tissues derived from stem segments of Actinidia polygama Miq. (matatabi or silver vine). Stem and petiole segments cultured on combined broad-leaved tree medium and woody plant medium (BW medium) supplemented with zeatin for 40 days formed many shoot buds. Callus tissues derived from the stem segments and subcultured on BW medium supplemented with 6-benzyladenine and 1-naphthaleneacetic acid formed shoot buds when the medium contained 13.7μM zeatin. BW medium containing low concentrations of sucrose strongly induced the formation of shoot buds from the callus.