Dual-Color Click Beetle Luciferase Heteroprotein Fragment Complementation Assays

β-catenin is a target for the ubiquitin–proteasome pathway

Hermann Aberle, Andreas Bauer, Jörg Stappert et al. · The EMBO Journal · 1997 · 2.5K citations · Full text

Protein Function, Molecular Regulation, Medicine +6

The two-hybrid system: a method to identify and clone genes for proteins that interact with a protein of interest.

Cheng‐Ting Chien, Paul L. Bartel, Rolf Sternglanz et al. · Proceedings of the National Academy of Sciences · 1991 · 1.5K citations

Genetics, Molecular Biology, Reporter Gene +22

Split ubiquitin as a sensor of protein interactions in vivo.

Nils Johnsson, Alexander Varshavsky · Proceedings of the National Academy of Sciences · 1994 · 781 citations

Protein Chemistry, Vivo Protein Interactions, Protein Function +13

Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis

Chang-Deng Hu, Tom K. Kerppola · Nature Biotechnology · 2003 · 781 citations · Full text

Living Cells, Immunocytochemical Technique, Engineering +13

Two distinct signal transmission pathways in T lymphocytes are inhibited by complexes formed between an immunophilin and either FK506 or rapamycin.

Barbara E. Bierer, Petri S. Mattila, Robert F. Standaert et al. · Proceedings of the National Academy of Sciences · 1990 · 708 citations · Full text

T-regulatory Cell, Dissociation Constant, Immune Regulation +19