Isolation and characterization of 12 tetranucleotide repeat microsatellite loci from the green‐backed tit (<i>Parus monticolus</i>)

MEI‐TING WANG, Yu-Cheng Hsu, Cheng‐Te Yao, Shou‐Hsien Li

Molecular Ecology Notes · 2005 · 31 citations · 11 references

Concepts

Abstract

Abstract From a partial genomic library enriched for GATA short tandem repeats, we developed 12 polymorphic microsatellite loci from the green‐backed tit ( Parus monticolus ). We characterized these loci by genotyping 30 adult individuals with unknown relationship. The number of alleles ranged from four to 17 per locus (mean = 9.3 alleles) and the observed heterozygosity for each locus ranged from 0.633 to 0.933 (mean = 0.789). All loci conformed to Hardy–Weinberg expectations. Four of 66 possible pairwise comparisons between loci showed significant gametic disequilibrium.

References

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