High-performance Liquid Chromatographic Determination of Paracetamol, Propyphenazone, and Caffeine in Pharmaceutical Formulations

Abstract

Abstract: Ethanolic extract of fruit and leaves of Coccinia indica revealed the presence of saponins. The purified fraction Ci from ethanolic extract by gradient silica gel column chromatography in the dose 25 mg/kg (Ci-1) and 50 mg/kg (Ci-2) (p.o.) showed significant dose dependent reduction in SGPT,SGOT, bilirubin, total protein, liver weight and lipid peroxide levels with reference to the standard, silymarin (25 mg/kg, p.o).The Ci compound also revealed significant dose dependent reduction in the hepatic antioxidant enzyme activities such as super oxide dismutase, 1 12 glutathione, catalase, and peroxidase. The structural characterization of Ci compound by microanalysis, UV, IR, 1H NMR, 13C NMR spectroscopy and Mass spectrometry revealed structure with molecular formula C29H50 O ( beta sitosterol ). Hepatoprotective potential of Ci 29 50 compound, beta sitosterol was inferred from it's antihepatotoxic activities on serum transaminases and hepatic antioxidant enzymes in CCl4 intoxicated rats.