Plant Disease · 1997 · 103 citations · 32 references
AgricultureBiologyPlant PathologyPlant VirologyTransgenic CropsVirologyDisease Resistance EvaluationPlant Disease ResistancePlant Virus ResistancePlant Virus SymptomatologyPlant VirusesResistance Breeding For Viral DiseasesDormancy StagesHost Resistance GenesPolymerase Chain Reaction (Pcr) AssaysTransgenic Virus ResistanceVirus Resistance BreedingVirus Resistance ScreeningVirus-resistant PlantsVirus-resistant Varieties
Transgenic plum trees (Prunus domestica) containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV by aphid feeding or chip budding. Infection was monitored by evaluation of virus symptoms, DAS-ELISA, and immunoblot assays. Based on observations and analyses over 3 years including two dormancy cycles, one out of five transgenic clones (C-5), was found to be resistant to infection whether inoculated by aphids or by chip budding. PPV could not be detected in any inoculated plants of the C-5 clone by immunoblot or immunocap-ture-reverse transcriptase-polymerase chain reaction assays. To our knowledge, this is the first P. domestica clone resistant to PPV infection produced by genetic engineering.
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