Gene expression profile of dermal mesenchymal stem cells from patients with psoriasis

Abstract

BACKGROUND: Mesenchymal stem cells (MSCs) are likely involved in pathological processes of immune-related diseases, including psoriasis because of their immunoregulatory and pro-angiogenic effects, and the vascular proliferation, angiectasis and perivascular lymphocyte infiltration are known to be predominantly responsible for the pathological alterations in psoriasis. OBJECTIVE: This study aimed to investigate the gene expression profile of dermal MSCs from patients with psoriasis. METHODS: We isolated and expanded dermal MSCs from psoriatic patients and normal controls by using the attachment assay and conducted mRNA expression profile and gene ontology analyses using microarray. RESULTS: The gene expression profile of MSCs from psoriatic derma was markedly different from the normal derma-derived MSCs; the angiogenesis-related genes such as vascular endothelial growth factor A, insulin-like growth factor-binding protein-5, and GATA6 showed significant differential expression. CONCLUSIONS: These results indicate that MSCs from the derma of psoriasis patients might be involved in the early development of psoriasis because of their pro-angiogenic potential as well as the immunoregulatory effect.