Muscle glycogen synthase translocates from the cell nucleus to the cytosol in response to glucose

Abstract

We have studied the intracellular localization of muscular glycogen synthase by fusing the green fluorescent protein (GFP) of the jelly-fish Aequorea victoria to the N-terminus of human muscle glycogen synthase (HMGS), and expressing the chimeric protein in C2C12, COS-1 cells, and primary cultured rat hepatocytes. In contrast to what we have recently found for the hepatic glycogen synthase (Fernandez-Novell et al. (1997) Biochem. J. 321, 227-231), the GFP/HMGS fusion protein is localized to the nucleus of the cell in the absence of glucose, and in the presence of the sugar it is essentially found in the cytosol. Insulin is not required for the translocation of the enzyme.