Dextran Sodium Sulfate Inhibition of Real-Time Polymerase Chain Reaction Amplification: A Poly-A Purification Solution

Abstract

DSS is a potent inhibitor of real-time qPCR amplification and interferes with tissue-specific gene expression analysis in DSS-exposed mice. Poly-A purification of tissue-derived RNA results in reliable and cost-effective gene expression analysis in DSS-exposed mice.