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The Study of 2,3,4-Trihydroxyacetophenone and 2,4,6-Trihydroxyacetophenone as Matrices for DNA Detection in Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry
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1996
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Dna AnalysisBiological Mass SpectrometryMolecular BiologySpectrochemical AnalysisAnalytical InstrumentationBioanalysisAnalytical ChemistryChromatographyBiochemistryOligonucleotideDna ReplicationDna DetectionBiomolecular EngineeringNatural SciencesSpectroscopyMass SpectrometryNucleic Acid AmplificationAmmonium Citrate MatrixMatrix-assisted Laser Desorption/ionizationMedicineDrug Analysis
The matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometric study of DNA detection using 2,3,4-trihydroxyacetophenone, 2,4,6-trihydroxyacetophenone, and their combination has been carried out systematically. The results show that a mixture of 2,4,6-trihydroxyacetophenone, 2,3,4-trihydroxyacetophenone and ammonium citrate with molar ratios of 2:1:1 serves as a good matrix for the detection of DNA, especially for samples containing a small quantity of DNA such as polymerase chain reaction product. The resolution and shot-to-shot reproducibility using this matrix are better than, and the MALDI sensitivity comparable to, that obtained when using 3-hydroxy picotinic acid (3-HPA), PA and ammonium citrate matrix (9:1:1). The mechanism of desorption/ionization using this matrix is discussed.