Real-Time Quantitative PCR: Standardized Detection of Minimal Residual Disease in Pediatric Acute Lymphoblastic Leukemia

Abstract

The IgH, TCR and TEL-AML1 markers can be used as targets by real-time PCR under the same cycling profile, allowing quantitation of MRD in more 95% of patients with pre-B ALL. This standardized, real-time PCR technique should simplify monitoring MRD in clinical trials.