PLANT PHYSIOLOGY · 2006 · 270 citations · 50 references
AgriculturePlant BiologyPlant SciencesPlant BiochemistryPlant BiotechnologyPlant-microbe InteractionsChemical Defense In PlantsPlant Defense ResponsesPlant Disease ResistancePlant Stress BiotechnologyPlant-pathogenic FungiStress BiochemistryAbiotic StressDefense-related EnzymesGene OverexpressionGene Overexpression StrategiesPathogen Resistance GenesPlant Resistance GenesPlant Resistance ResponsesTransgenic Plants
Genes encoding defense-related proteins have been used to alter the resistance of plants to pathogens and other environmental challenges, but no single fungal gene overexpression has produced broad-spectrum stress resistance in transgenic lines. We have generated transgenic tobacco (Nicotiana tabacum) lines that overexpress the endochitinases CHIT33 and CHIT42 from the mycoparasitic fungus Trichoderma harzianum and have evaluated their tolerance to biotic and abiotic stress. Both CHIT33 and CHIT42, individually, conferred broad resistance to fungal and bacterial pathogens, salinity, and heavy metals. Such broad-range protective effects came off with no obvious detrimental effect on the growth of tobacco plants.
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A Simple and General Method for Transferring Genes into Plants
R. Horsch, J. E. Fry, Nancy Hoffmann et al. · Science · 1985 · 4.8K citations
John Ryals, Urs Neuenschwander, M. G. Willits et al. · The Plant Cell · 1996 · 2K citations · Full text