Carcinogenesis · 1993 · 32 citations · 0 references
PharmacyOncologyOral BiologyCarcinogenesisDna RepairOral OncologyDna DamageDna Double-strand Break RepairDna Strand BreaksGenotoxic CarcinogenesisOral Cancer BiomarkersOral CarcinogenesisDna Break BiomarkersDna Double-strand BreaksDna Oxidative AdductsDna Strand Break AnalysisDna Strand Break RepairDouble-strand Dna BreaksIonizing Radiation-induced Dna BreaksOral Cancer Incidence
In lymphocytes of 12 oral cancer patients (and two control groups) the frequencies of DNA single-strand breaks and DNA-protein cross-linking were determined by alkaline filter elution. We found elevated DNA elution rates, which must be interpreted as an increased strand breakage frequency. There were significant correlations between the DNA strand breakage frequency and smoking habits. Using the 32P-postlabelling assay we determined the DNA adduct level in lymphocytes of 23 oral cancer patients (and two control groups). No significant influence of smoking habit on the DNA adduct level could be detected. There was a significant correlation between the DNA adduct level and the gamma-glutamyltranspeptidase (GGT) value, suggesting systemic influences of alcohol drinking habits on the adduct level.