Prenatal Diagnosis · 1993 · 52 citations · 17 references
MedicineMicrobiologyObstetricsDiagnostic MicrobiologyPrenatal DiagnosisVirologyFetal Infection DiagnosisPerinatal InfectionPregnancy DiagnosisPrenatal Molecular TestingViral DiagnosticsViral InfectionsAmniotic Fluid InfectionChorionic Villi AbnormalitiesInfections In PregnancyMaternal-fetal Infection InvestigationPolymerase Chain Reaction (Pcr) AmplificationPolymerase Chain Reaction DetectionPrenatal Infection DiagnosisPrenatal Viral Infection
Eight patients were referred for prenatal diagnosis for suspected fetal cytomegalovirus infection (CMV): six for documented first-trimester infection and two for abnormal ultrasound evaluation suggestive of fetal infection. Three methods of diagnosis were employed: (1) amniotic fluid viral cultures and CMV-specific IgM in fetal serum; (2) amniotic fluid cultures and detection by polymerase chain reaction amplification of CMV-specific DNA in chorionic villi; and (3) detection of CMV-specific DNA in villus samples only. Amniotic fluid cultures detected all cases of infection, but CMV-specific IgM was not a reliable indicator of infection in any case. DNA analysis correlated well with both culture results and clinical outcome.
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