Cytogenetics and Cell Genetics · 2008 · 29 citations · 0 references
Life SciencesGeneticsMolecular GeneticsComparative GenomicsGenomicsHuman GeneticsComparative Genomic HybridizationHybridizationVariant DetectionAmplificationAmplification SpecificityDetection Limit AssessmentDetection Limit EstimationDna Hybridization AssaysGenomic Amplification DetectionMethod Detection LimitOverrepresented Sequence Detection
Artificial amplicons of known size, constructed by use of YACs featuring human 8p12 and 12q13, were analyzed by comparative genomic hybridization (CGH). A minimum of 15 Mb of overrepresented DNA sequences could be detected. The sensitivity is (1) not dependent on the chromosome site and (2) related to the size of the amplicon, decreasing with decreasing size.