In vitro antioxidant activities of jelly fish Chrysaora quinquecirrha venom from southeast coast of India

Abstract

To extract the venom from Chrysaora quinquecirrha (C. quinquecirrha) and tested for antioxidant potential of both crude and fractions. These venom proteins were purified through DEAE- cellulose column chromatography. The protein content from crude and fractionated samples (Frc 1 to Frc 3) showed 398, 180, 203 and 286 μg/mL respectively. The molecular weight of purified protein were determined by SDS- PAGE and we found five distinct bands with molecular weight of 20, 35, 45, 70, 74 and 100 kDa in the crude 70 and 100 kDa in Frc-1, 21 and 68 kDa in Frc-2 and 14, 65 and 104 kDa in Frc-3. The in vitro antioxidant activity of crude and purified samples were evaluated in different assay systems Viz. radical scavenging activity by DPPH reaction, Superoxide radical scavenging activity in PMS/NADH-NBT system, hydroxyl radical by Fe3+-Ascorbate- EDTA, H2O2 system and Nitric oxide (NO) activity in sodium nitroprusside/Greiss Reagent system. Fre-3 displayed the maximal antioxidant activity from all the assay system tested. Inhibitory activities in DPPH radicals 15%-92% Superoxide radicals 21%-96% hydroxyl radicals 30%-95% and Nitric oxide activity also showed significant activities. These results indicate that the jelly fish C. quinquecirrha venom have remarkable antioxidant activities. Further studies will fulfill for purification and structural elucidation.