BMC Cell Biology · 2008 · 51 citations · 48 references
Life SciencesBiochemistryCell BiologyMembrane TraffickingProteomicsSignal TransductionFunctional ProteomicsLysosome BiologyMass Spectrometry-based ProteomicsProteomic ProfilingSecretory PathwaySubcellular ProteomicsMass Spectrometry CharacterizationMembrane Trafficking ProteinsMembrane Trafficking To Secretory GranulesPeptide Mass SpectrometryPeptide-mass FingerprintingProteomic ClassificationProteomic Data AnalysisProteomic Identification
BACKGROUND: Lamellar bodies are lysosome-related secretory granules and store lung surfactant in alveolar type II cells. To better understand the mechanisms of surfactant secretion, we carried out proteomic analyses of lamellar bodies isolated from rat lungs. RESULTS: With peptide mass fingerprinting by Matrix Assisted Laser Desorption/Ionization - Time of Flight mass spectrometry, 44 proteins were identified with high confidence. These proteins fell into diverse functional categories: surfactant-related, membrane trafficking, calcium binding, signal transduction, cell structure, ion channels, protein processing and miscellaneous. Selected proteins were verified by Western blot and immunohistochemistry. CONCLUSION: This proteomic profiling of lamellar bodies provides a basis for further investigations of functional roles of the identified proteins in lamellar body biogenesis and surfactant secretion.
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A genomic perspective on membrane compartment organization
Jason B. Bock, Hugo Matern, Andrew A. Peden et al. · Nature · 2001 · 692 citations · Full text
Modulation of gene expression by calreticulin binding to the glucocorticoid receptor
Kimberly Burns, Brenda Duggan, Eric A. Atkinson et al. · Nature · 1994 · 395 citations