International Journal of Pest Management · 1994 · 44 citations · 11 references
AgricultureCrop SciencesPlant SciencesPlant VirologyPlant-microbe InteractionsVirologyDisease DynamicsPlant Virus DetectionPlant Virus Host RangePlant Virus TransmissionPlant VirusesVirus EpidemiologyDouble Antibody Sandwich AssaysEpidemiology Of Virus SpreadNucleic Acid Hybridization AssaysPlant Viral PathogenesisViral Diseases Of CropsVirus Detection AssaysVirus DiseasesWeed-associated Viral Diseases
Abstract Solanum viarum Dunal (tropical soda apple), an introduced and rapidly spreading weed, currently infests over 60 000 ha in Florida. Approximately 220 plants were sampled in seven stands of S. viarum in south‐west and west central Florida during 1992 and 1993 to determine the occurrence of nine viruses which can infect solanaceous crops. Virus detection utilized a double antibody sandwich‐enzyme linked immunosorbent assay, or a nucleic acid spot hybridization assay. The viruses detected included cucumber mosaic virus (CMV), potato leaf roll virus (PLRV), potato virus Y (PVY), tobacco etch virus (TEV), tomato mosaic virus (ToMV), and tomato mottle virus (TMoV). Transmission of PVY and ToMV from S. viarum produced a range of symptoms in pepper, tobacco, and tomato. Isolates of TEV from pepper, and PVY and TMoV from tomato were transmitted to S. viarum. Solanum viarum was also naturally infected in the field by Altemaria solani Sorauer and infested by Colorado potato beetles (Leptinotarsa decenlineata Say).
11
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Randall K. Saiki, David H. Gelfand, Susanne Stoffel et al. · Science · 1988 · 17.2K citations
A rapid DNA isolation procedure for small amounts of fresh leaf tissue
JJ Doyle, JL Doyle, J.J. Doyle et al. · Medical Entomology and Zoology · 1987 · 2.4K citations