A modified regeneration method for streptomycete protoplasts.

Abstract

In 1974, we1} reported on the culture media and conditions for preparing stable protoplasts of streptomycetes and the procedures for reverting them at high frequency to a filamentous state by plating the surface of the synthetic agar media (Rl and R2). This method, sometimes with minor modifications, has been employed by many researchers on protoplast fusion2~7) or genetic transformation,8~n) including recombinant DNAexperiments,