Rapid Communications in Mass Spectrometry · 2004 · 72 citations · 5 references
PharmacyPharmaceutical AnalysisAnalytical ChemistryChromatographic AnalysisMass Spectrometric AnalysisInstrumental AnalysisIonization TechniquesLiquid ChromatographyMass SpectrometryMetabolite QuantificationBioanalytical ApplicationsElectrospray IonizationIon SuppressionIonizationLiquid Chromatography-mass SpectrometryLiquid Chromatography-mass Spectrometry AnalysisSuppressionTandem Mass Spectrometry
Ion suppression is a well-known phenomenon in electrospray ionization (ESI) mass spectrometry. These suppression effects have been shown to adversely affect the accuracy and precision of quantitative bioanalytical methods using ion spray. Such suppression effects have not been as well defined in atmospheric pressure chemical ionization (APCI) and there is some debate whether these effects actually occur in the ionization process using APCI. Here an example is described where clear ion suppression was observed during studies on a model compound and three metabolites using APCI liquid chromatography/tandem mass spectrometry (LC/MS/MS).
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