Switchable boronate affinity materials for thermally modulated capture, separation and enrichment of cis-diol biomolecules

Abstract

A new thermoresponsive boronate affinity chromatographic material, P(N-isopropylacrylamide-4-vinylphenylboronic acid) P(NIPAAm-co-VPBA) grafted silica featuring a thermally switchable property, was prepared and successfully employed for the specific capture–release of cis-diol biomolecules with simpler adjustment to the temperature instead of the conventional need to change the pH of the mobile phase.