FEBS Letters · 1992 · 109 citations · 32 references
Life SciencesBiochemistryMolecular BiologyCell SignalingElectrophysiologySignal TransductionCalcium SignalingCardiac Ion ChannelsIon Channel PhysiologyIon Channel RegulationIon Channel SignalingIon ChannelsCalcium Channel PhysiologyCalcium Signaling Through Ion ChannelsCardiac Calcium HandlingCardiac Ion Channel RegulationPhosphorylation CascadesPolyclonal Antibody AssaysPolyclonal Antibody RecognitionSecond Messenger Regulation Of Ion Channels
A polyclonal antibody, CR2, prepared using the C-terminal peptide of the alpha 1 subunit of the rabbit cardiac DHP-sensitive Ca channel, specifically immunoprecipitated the [3H]PN200-110-labeled Ca channel solubilized from cardiac microsomes. The antibody recognized 250 and 200-kDa cardiac microsomal proteins as determined by immunoblotting, and cAMP-dependent protein kinase phosphorylated the 250-kDa, but not the 200-kDa protein in vitro. CHO cells, transfected with the cardiac alpha 1 subunit cDNA carried by an expression vector, synthesized a 250-kDa protein which was recognized by CR2. Adding db-cAMP or forskolin to the transformed CHO cells induced phosphorylation of the 250-kDa protein and stimulated the DHP-sensitive Ba current under patch-clamp conditions. These results suggested that the cardiac DHP-sensitive Ca channel was regulated by cAMP-dependent phosphorylation of the alpha 1 subunit.
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Vectors for selective expression of cloned DNAs by T7 RNA polymerase
Alan H. Rosenberg, Barbara N. Lade, Chui Dao-shan et al. · Gene · 1987 · 1.5K citations
Primary structure of the receptor for calcium channel blockers from skeletal muscle
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