Molecular Plant-Microbe Interactions · 2001 · 134 citations · 23 references
Life SciencesGeneticsMicrobiologyGene ExpressionGene Expression AnalysisGene FunctionBacterial Gene RegulationGene Expression RegulationGene Regulation In MicrobesPlasmid BiologyPlasmid EngineeringPlasmid GeneticsBacterial Expression VectorsBacterial Plasmid ConstructionBacterial PromotersDna Cloning VectorsGene Cloning VectorsGene Regulation In BacteriaRhizobium SymbiosisSymbiosis Plasmids
The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported. Plasmid stability is conferred by the RK2 par locus or by insertion of the spsAB or spsCD symbiotic plasmid stability loci from pNGR234a of Rhizobium sp. NGR234. The vectors carry multiple cloning sites with protection against read-through transcriptional activity of vector sequences. Vector derivatives with the constitutive nptII promoter or a promoterless gusA gene are suitable for the study of gene function or regulation in bacteria.
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Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria
N. T. Keen, Stanley Tamaki, Daisuke Kobayashi et al. · Gene · 1988 · 1.5K citations
Molecular basis of symbiosis between Rhizobium and legumes
Christoph Freiberg, R. Fellay, Amos Bairoch et al. · Nature · 1997 · 761 citations