Investigation of Enantiomer Bonding on a Chiral Stationary Phase by FT-Raman Spectrometry

Abstract

The bonding of serine, phenylalanine, and mandelic acid enantiomers on an N-3,5-dinitrobenzoyl-l-leucine chiral stationary phase (on zeolite A support) has been investigated by FT-Raman spectrometry. It was found that retention is due to hydrogen bonds and π-stacking interactions between the stationary phase and the analyte. The involvement of the two different amide groups (as donor and/or acceptor) in the complexation reaction can be followed based on spectral data. A correlation was found between the ratio of the amide I and the ring stretching (1532 cm-1) bands and retention data.