Animal Genetics · 1993 · 29 citations · 6 references
Life SciencesGeneticsMolecular GeneticsEndocrine GeneticsHormone BiochemistryReproductive GeneticsGrowth HormoneRestriction Enzyme TechnologySheep ReproductionPolymerase Chain Reaction-restriction AnalysisRestriction Fragment AnalysisRestriction Fragment Length Polymorphism AnalysisRestriction Fragment Length Polymorphism MappingRestriction Site Polymorphism
PvuII restriction fragment length polymorphism (RFLP) was found at the growth hormone locus in sheep carrying the GH2 allele where the gene is duplicated. By restriction analysis and using the polymerase chain reaction we demonstrated that this RFLP is due to a mutation at the PvuII site located in the second intron of the 3' copy of the GH2 allele.
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