A Clinical Biomarker Assay for The Quantification of D3-Creatinine and Creatinine Using LC–MS/MS

Abstract

Novel LC-MS/MS assays were developed that quantify the biomarkers d3-creatinine, creatinine and d3-creatine in urine. This method allows the estimation of total body creatine pool size and subsequent calculation of muscle mass. This assay was originally validated as fit-for-purpose and was followed by full validation.