Analysis of Global Expression Profiles of <i>Arabidopsis</i> Genes Under Abscisic Acid and H₂O₂ Applications

Abstract

Abstract To gain insight into the coordination of gene expression profiles under abscisic acid (ABA) and H 2 O 2 applications, global changes in gene expression in response to ABA and H 2 O 2 in Arabidopsis seedlings were investigated using GeneChip (Santa Clara, CA, USA) arrays. Among over 24 000 genes present in the arrays, 459 transcripts were found to be significantly increased, whereas another 221 decreased following H 2 O 2 treatment compared with control. Similar to treatment with H 2 O 2 , ABA treatment elevated the transcription of 391 genes and repressed that of 322 genes. One hundred and forty‐three upregulated genes and 75 downregulated genes were shared between the two treatments and these genes were mainly involved in metabolism, signal transduction, transcription, defense, and resistance. Only two genes, which encode an APETALA2/dehydration‐responsive element binding protein (AP2/DREBP) family transcriptional factor and a late embryogenesis‐abundant protein, were downregulated by H 2 O 2 , but upregulated by ABA. These results suggest that, similar to ABA, H 2 O 2 plays a global role in gene transcription of Arabidopsis seedlings. The transcriptional responses induced by the application of exogenous ABA and H 2 O 2 overlapped substantially. These two treatments regulated most of the downstream genes in a coordinated manner. (Managing editor: Li‐Hui Zhao)