FEBS Letters · 2004 · 183 citations · 17 references
Life SciencesPlant BiologyPlant SciencesPlant BiochemistryPlant PhysiologySignal TransductionCalcium SignalingCyclic Nucleotide SignalingSalinity PhysiologySalinity Stress PhysiologySalt Stress PhysiologyStress BiochemistryCyclic Guanosine Monophosphate PathwayCyclic Guanosine Monophosphate SignalingGuanylyl Cyclase RegulationMolecular Responses To Salt StressOsmotic StressOsmotic Stress ResponseSalt Stress ResponsesSalt Stress Signaling Pathways
A guanylyl cyclase has been recently identified in Arabidopsis but, despite the use of pharmacological inhibitors to infer roles of the second messenger 3',5'-cyclic guanosine monophosphate (cGMP), very few measurements of actual cGMP levels in plants are available. Here, we demonstrate that cGMP levels in Arabidopsis seedlings increase rapidly (< or =5 s) and to different degrees after salt and osmotic stress, and that the increases are prevented by treatment with LY, an inhibitor of soluble guanylyl cyclases. In addition, we provide evidence to suggest that salt stress activates two cGMP signalling pathways - an osmotic, calcium-independent pathway and an ionic, calcium-dependent pathway.
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