Rapid Quantification of Biotin in Human Skin Extracts After Dermal Application Using High-performance Liquid Chromatography–Electrospray Mass Spectrometry

Abstract

A method for the determination of the vitamin biotin in extracts from different layers of human skin is presented, which has a detection limit of 800 pg ml–1 without further sample preparation. The quantification was performed using an HPLC procedure on a reversed phase column, which takes only 4 min, coupled to a single quadrupole mass spectrometer via an electrospray ionization interface. The detection was performed in the positive selected ion monitoring mode. Additional MS–MS and MSn experiments with biotin using an ion trap mass spectrometer are described.