Journal of the American Society for Mass Spectrometry · 1998 · 117 citations · 30 references
Life SciencesBiochemistryMolecular BiologyAnalytical ChemistryMass Spectrometric AnalysisProteomicsMass SpectrometryMass Spectrometry Data AnalysisMass Spectrometry-based ProteomicsPeptide AnalysisPeptide IdentificationProtein SeparationCharge-to-mass RatioIon Mobility SpectrometryPeptide Charge State AnalysisPeptide Mass SpectrometryPeptide SeparationProtein Mass SpectrometryTime-of-flight Mass AnalyzersTime-of-flight Mass Spectrometry
A mixture of peptides from a complete tryptic digest of ubiquitin has been analyzed by ion mobility/time-of-flight mass spectrometry techniques. All components of the mixture were electrosprayed and ions were separated in the gas phase based on differences in their mobilities through helium before being dispersed into a time-of-flight mass spectrometer for mass-to-charge analysis. The data show that ions separate into families primarily according to differences in their charge states and, to a lesser extent, differences in conformation. This approach reduces spectral congestion typically associated with electrosprayed mixtures and provides charge assignments for mass-to-charge ratio data. Gas-phase separations of ions appear to provide a new physical basis for characterizing components of biological mixtures.
30
Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry
Matthias Wilm, Tony Houthaeve, Stephen Breit et al. · Nature · 1996 · 1.7K citations