Novel Stationary Phases for Affinity Chromatography. Nucleobase-Selective Recognition of Nucleosides and Nucleotides on Poly(9-vinyladenine)-Supported Silica Gel

Abstract

Abstract Poly(9-vinyladenine) (PVAd) was immobilized or coated on macroporous silica gel to be used as a stationary phase for HPLAC of nucleosides and nucleotides. The stationary phases showed nucleobase-selective recognition ability for nucleic acid constituents and gave practically useful HPLAC columns. On the basis of the results, the relationships between separation ability and the structure of PVAd are discussed.