Basolateral amygdala noradrenergic influence enables enhancement of memory consolidation induced by hippocampal glucocorticoid receptor activation

TLDR

Lesions of the basolateral amygdala block glucocorticoid‑induced enhancement of inhibitory avoidance memory. This study examines how the basolateral amygdala permits hippocampal glucocorticoid‑mediated memory consolidation. Unilateral hippocampal glucocorticoid receptor activation dose‑dependently increases 48‑h inhibitory avoidance retention, but this effect is blocked by β‑adrenergic antagonism in the ipsilateral BLA, while muscarinic blockade does not interfere. The results confirm that β‑adrenergic activity in the BLA is essential for glucocorticoid‑induced memory consolidation and that the interaction is ipsilateral, indicating neural pathway mediation rather than peripheral stress.

Abstract

Previously, we reported that bilateral excitotoxic lesions of the basolateral nucleus of the amygdala (BLA) block the enhancing effects of posttraining systemic or intrahippocampal glucocorticoid administration on memory for inhibitory avoidance training. The present study further examined the basis of this permissive influence of the BLA on hippocampal memory functioning. Immediate posttraining unilateral infusions of the specific glucocorticoid receptor agonist RU 28362 (11β,17β-dihydroxy-6,21-dimethyl-17α-pregna-4,6-trien-20-yn-3-one; 3.0, 10.0, or 30.0 ng in 0.5 μl) administered into the dorsal hippocampus of male Sprague–Dawley rats induced dose-dependent enhancement of 48-h inhibitory avoidance retention. Infusions of the β-adrenoceptor antagonist atenolol (0.5 μg in 0.2 μl) into the ipsilateral, but not the contralateral, BLA 10 min prior to training blocked the hippocampal glucocorticoid effects on memory consolidation. Infusions of the muscarinic cholinergic antagonist atropine (0.5 μg in 0.2 μl) into either the ipsilateral or contralateral BLA before training did not block the hippocampal glucocorticoid effects. These findings provide further evidence that β-adrenergic activity in the BLA is essential in enabling glucocorticoid-induced modulation of memory consolidation and are consistent with the hypothesis that the BLA regulates the strength of memory consolidation in other brain structures. The ipsilateral nature of the BLA–hippocampus interaction indicates that BLA influences on hippocampal memory processes are mediated through neural pathways rather than by influences by means of the activation of peripheral stress responses.