A Chemoenzymatic Route to Diversify Aminolgycosides Enables a Microarray‐Based Method to Probe Acetyltransferase Activity

Abstract

Chemoenzymatic synthesis: A chemoenzymatic route for modification of aminoglycosides is disclosed. The significant feature of this approach is the discovery that an aminoglycoside 3-N-acetyltransferase, AAC(3)-IV, from Escherichia coli accepts azidoacetyl coenzyme A (AzAcCoA) as a substrate that can be further chemically diversified. This approach was used to develop a carbohydrate microarray method to study modification of aminoglycosides. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.