Journal of Applied Polymer Science · 1990 · 32 citations · 6 references
Life SciencesBiochemistryBiotechnologyBiocatalysisEnzymologyPolymer ChemistryEnzyme CharacterizationEnzyme ImmobilizationEnzyme IsolationEnzyme PurificationImmobilized EnzymesPolymerization ProcessesBinding CapacityCrosslinked PolymersEnzymatic PolymerizationEnzyme CapacityPolymer Composition EffectsPolymer Matrix CompositionPorous Support MaterialsSuspension Polymerization
Abstract Porous beads have been prepared by suspension polymerization of acrylic monomers and divinyl benzene in the presence of a porogen. The porosity varies greatly with the polymer composition, with the amount and type of porogen and with the conditions for the preparation. The enzyme binding capacity and activity has been determined for a lipase. The best results are found for crosslinked poly(methyl methacrylate) beads while other polyacrylates are less effective. Compositions based on poly(methacrylic acid) are not suitable for enzyme binding.
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