Evaluation of 4-Nitrophenol ELISA Kit for Assessing the Origin of Organic Pollution in Wastewater Treatment Works

Marinella Farré, Anna Oubiña, M.‐Pilar Marco, Antoni Ginebreda, Lluís Tirapu, ‪Damià Barceló

Environmental Science & Technology · 1999 · 21 citations · 12 references

Concepts

Abstract

A cost-effective strategy based on a recently developed ELISA for 4-nitrophenol was used for the characterization of wastewater samples (industrial and/or urban) of the primary sewage effluent and secondary sewage effluent, also called influent and effluent, respectively, of wastewater treatment works (WWTW) using either biological treatment with secondary settlement and/or physicochemical treatment. Two of the WWTW received only domestic wastewaters, whereas three of them received 60−70% of industrial effluents that were mixed with domestic wastewaters before entering WWTW. The 4-nitrophenol ELISA kit was used as a parameter to evaluate the performance of the treatment plants by comparing the ELISA measurements with those routinely used in WWTW, such as total organic carbon and total phenols content using 4-aminoantipyrine. The 4-nitrophenol ELISA gave a positive response to different wastewaters being a useful measurement for the estimation of the performance of the WWTW. The response obtained with 4-nitrophenol ELISA can differentiate the wastewater pollution discharged into WWTW, either from domestic or industrial sources.

References

12