Plant and Cell Physiology · 1997 · 120 citations · 0 references
Life SciencesPlant BiologyPlant SciencesEnzymologyPlant BiochemistryPlant Cell BiologyCell GrowthCell Wall BiochemistryCell Wall BiosynthesisCell Wall PhysiologyPlant CellsPlant EnzymesCell Elongation RegulationCellulose AccumulationCellulose Biosynthesis PathwayCellulose Biosynthesis RegulationPlant Cell Wall CellulosePlant Cell Wall-degrading EnzymesReverse Transcription Polymerase Chain Reaction (Rt-pcr)Secondary Cell Wall Deposition
mRNAs for cell wall-related enzymes in developing cotton fiber cells were measured by reverse transcription-PCR analysis. Both endo-1,4-beta-glucanase and expansin mRNA levels were high during cell elongation but decreased when cell elongation ceased, and xyloglucan decreased. The endo-1,3-beta-glucanase mRNA level was very low in the elongating cells but increased gradually at the onset of secondary wall synthesis, accompanying the massive deposition of cellulose. Endoxyloglucan transferase and sucrose synthase mRNA levels were constant during all stages of growth.