Molecular characterization of weak D phenotypes by site‐directed mutagenesis and expression of mutant Rh–green fluorescence protein fusions in K562 cells

Abstract

The mutations--G212C (new weak D type), V270G (weak D type 1) and G358A (type 2)-- in transmembranous regions had obvious effects on the D epitopes recognized by mAbs. The results of this study provide direct evidence that these mutations can account for weak D phenotypes.