A Novel Method of Cellular Labeling: Anchoring MR‐Imaging Reporter Particles on the Outer Cell Surface

Abstract

Cellular labeling. MR visualization of cells has been attained by anchoring negatively charged Gd-loaded nanoparticles at the cellular membrane by a polycationic linker. Each nanoparticle contains approximately 800 Gd-chelates and each cell has been loaded with approximately 5×106 particles. In this way only 15 % of the negative charges on the membrane has been used. The method has been validated by confocal microscopy.