Construction of a [NiFe]-hydrogenase deletion mutant of <i>Desulfovibrio vulgaris</i> Hildenborough

Abstract

A mutant of Desulfovibrio vulgaris Hildenborough lacking a gene for [NiFe] hydrogenase was generated. Growth studies, performed for the mutant in comparison with the wild-type, showed no strong differences during the exponential growth phase. However, the mutant cells died more rapidly in the stationary growth phase.